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Fhl2-HA-P2A-mitomeGFP
(Plasmid #225231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225231 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Addgene plasmid 225270
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fhl2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Fhl2 (a.k.a. SLIM 3)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA-P2A-mitomeGFP- (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PspOMI (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Fhl2 gene was amplified from a plasmid reported in the following publication: Basu, H., Pekkurnaz, G., Falk, J., Wei, W., Chin, M., Steen, J., and Schwarz, T.L. (2021). Fhl2 anchors mitochondria to actin and adapts mitochondrial dynamics to glucose supply. Journal of Cell Biology 220. https://doi.org/10.1083/jcb.201912077.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fhl2-HA-P2A-mitomeGFP was a gift from Thomas Schwarz (Addgene plasmid # 225231 ; http://n2t.net/addgene:225231 ; RRID:Addgene_225231)

  • For your References section:

    Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1. Falk JE, Henke T, Gowrisankaran S, Wanderoy S, Basu H, Greally S, Steen J, Schwarz TL. J Cell Biol. 2026 Apr 6;225(4):e202501023. doi: 10.1083/jcb.202501023. Epub 2026 Jan 30. 10.1083/jcb.202501023 PubMed 41615403
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