pEF-ENTR-LUC (SN1)
(Plasmid
#22524)
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 22524-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 22524-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepENTR1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4058
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly Luciferase
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Insert Size (bp)1652
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RV (destroyed during cloning)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7forw
- 3′ sequencing primer ENTRrev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFirefly luciferase was derived from pGL3 plasmid (Promega). EF1a promoter was derived from pTracer-Bsd plasmid (Invitrogen).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector made by Stefania Nicoli (University of Massachusetts Medical School).
DNA (Catalog # 22524-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 22524-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-ENTR-LUC (SN1) was a gift from Eric Campeau (Addgene plasmid # 22524 ; http://n2t.net/addgene:22524 ; RRID:Addgene_22524)
Map uploaded by the depositor.
