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Addgene

LIG4 HAL-T2A-HYG-SV40pA-HAR
(Plasmid #225358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 2221
  • Total vector size (bp) 5445
  • Vector type
    CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-HYG-SV40pA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3224

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert designed by the Jagannathan lab. Plasmid synthesized and received from Twist Bioscience.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIG4 HAL-T2A-HYG-SV40pA-HAR was a gift from Sujatha Jagannathan (Addgene plasmid # 225358 ; http://n2t.net/addgene:225358 ; RRID:Addgene_225358)

  • For your References section:

    SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through Selectable Homology-Directed DNA Repair. Cortazar MA, Jagannathan S. Methods Mol Biol. 2025;2863:397-417. doi: 10.1007/978-1-0716-4176-7_23. 10.1007/978-1-0716-4176-7_23 PubMed 39535722
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