Skip to main content
Addgene

pX459_LIG4_Exon3
(Plasmid #225363)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pX459
  • Backbone size w/o insert (bp) 9152
  • Total vector size (bp) 9172
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LIG4
  • gRNA/shRNA sequence
    GCATAATGTCACTACAGATC
  • Species
    H. sapiens (human)
  • Entrez Gene
    LIG4 (a.k.a. LIG4S)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This protospacer sequence was previously defined by others (doi:10.1038/s41586-018-0461-z).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459_LIG4_Exon3 was a gift from Sujatha Jagannathan (Addgene plasmid # 225363 ; http://n2t.net/addgene:225363 ; RRID:Addgene_225363)

  • For your References section:

    SelectRepair Knockout: Efficient PTC-Free Gene Knockout Through Selectable Homology-Directed DNA Repair. Cortazar MA, Jagannathan S. Methods Mol Biol. 2025;2863:397-417. doi: 10.1007/978-1-0716-4176-7_23. 10.1007/978-1-0716-4176-7_23 PubMed 39535722
Related items: