pLenti6.3/TO/V5-DEST-Lifeact-mScarlet-I
(Plasmid
#225495)
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PurposeExpression of Lifeact-mScarlet-I (lentiviral vector)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti6.3/TO/V5-DEST
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLifeact
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Tag
/ Fusion Protein
- mScarlet-I
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer cmv
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.30.615654 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.3/TO/V5-DEST-Lifeact-mScarlet-I was a gift from Guillaume Jacquemet (Addgene plasmid # 225495 ; http://n2t.net/addgene:225495 ; RRID:Addgene_225495) -
For your References section:
Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers. Follain G, Ghimire S, Pylvanainen JW, Vaitkeviciute M, Hidalgo-Cenalmor I, Wurzinger D, Guzman C, Conway JRW, Dibus M, Harkonen J, Oikari S, Rilla K, Salmi M, Ivaska J, Jacquemet G. EMBO J. 2026 Jan 6. doi: 10.1038/s44318-025-00678-9. 10.1038/s44318-025-00678-9 PubMed 41495248