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pENTR2b-Lifeact-mNeonGreen
(Plasmid #225496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225496 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pENTR2b
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lifeact
  • Tag / Fusion Protein
    • mNeonGreen

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer cmv
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

First, gene blocks containing the lifeact sequence (Riedl et al., 2008) in frame with mNeonGreen (Shaner et al., 2013) and flanked with Kpn1 and Xho1 restriction sites were purchased from IDT (Integrated DNA Technologies, Inc.). Through enzymatic digestion (Kpn1, ThermoFisher Scientific, FD0524, and XhoI, ThermoFisher Scientific, FD0694) of the two sites on both gene blocks and the pENTR2b entry vector (ThermoFisher Scientific, A10463), we obtained pENTR2b-Lifeact-mNeonGreen.

Please visit https://doi.org/10.1101/2024.09.30.615654 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR2b-Lifeact-mNeonGreen was a gift from Guillaume Jacquemet (Addgene plasmid # 225496 ; http://n2t.net/addgene:225496 ; RRID:Addgene_225496)

  • For your References section:

    Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers. Follain G, Ghimire S, Pylvanainen JW, Vaitkeviciute M, Hidalgo-Cenalmor I, Wurzinger D, Guzman C, Conway JRW, Dibus M, Harkonen J, Oikari S, Rilla K, Salmi M, Ivaska J, Jacquemet G. EMBO J. 2026 Jan 6. doi: 10.1038/s44318-025-00678-9. 10.1038/s44318-025-00678-9 PubMed 41495248
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