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Addgene

pGCS-3xHA-TMEM230
(Plasmid #225512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGS (Addgene #85719)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMEM230
  • Species
    H. sapiens (human)
  • Entrez Gene
    TMEM230 (a.k.a. C20orf30, HSPC274, dJ1116H23.2.1)
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hai-Ning Du, backbone used is Addgene #85719.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.07.636106 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGCS-3xHA-TMEM230 was a gift from Wade Harper (Addgene plasmid # 225512 ; http://n2t.net/addgene:225512 ; RRID:Addgene_225512)

  • For your References section:

    EndoMAP.v1 charts the structural landscape of human early endosome complexes. Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, Walter JC, Harper JW. Nature. 2025 Jul;643(8070):252-261. doi: 10.1038/s41586-025-09059-y. Epub 2025 May 28. 10.1038/s41586-025-09059-y PubMed 40437099
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