pET28a(+)-8HisG2K
(Plasmid
#225540)
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Purpose(Empty Backbone) Empty backbone for bacterial expression. 8xHis tag, K in position 2 to minimise posttranslational gluconoylation.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET-28a(+)
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Backbone manufacturerNovagen
- Backbone size (bp) 5369
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Modifications to backbone8xHis, G2K mutation
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- 8xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a(+)-8HisG2K was a gift from Gregory Challis (Addgene plasmid # 225540 ; http://n2t.net/addgene:225540 ; RRID:Addgene_225540)
Map uploaded by the depositor.
