pEND-351_Cacnes_dCas9_CRISPRi
(Plasmid
#225617)
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PurposeCutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEND-350
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
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Selectable markersErythromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9
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SpeciesS. pyogenes
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MutationCodon optimized for Cutibacterium acnes
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEND-351_Cacnes_dCas9_CRISPRi was a gift from Marc Güell (Addgene plasmid # 225617 ; http://n2t.net/addgene:225617 ; RRID:Addgene_225617) -
For your References section:
Synthetically programmed antioxidant delivery by a domesticated skin commensal. Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M. Cell Syst. 2025 Feb 19;16(2):101169. doi: 10.1016/j.cels.2025.101169. Epub 2025 Feb 6. 10.1016/j.cels.2025.101169 PubMed 39919749