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pEND-351_Cacnes_dCas9_CRISPRi
(Plasmid #225617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEND-350
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    S. pyogenes
  • Mutation
    Codon optimized for Cutibacterium acnes
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEND-351_Cacnes_dCas9_CRISPRi was a gift from Marc Güell (Addgene plasmid # 225617 ; http://n2t.net/addgene:225617 ; RRID:Addgene_225617)

  • For your References section:

    Synthetically programmed antioxidant delivery by a domesticated skin commensal. Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M. Cell Syst. 2025 Feb 19;16(2):101169. doi: 10.1016/j.cels.2025.101169. Epub 2025 Feb 6. 10.1016/j.cels.2025.101169 PubMed 39919749
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