pEMS2367
(Plasmid
#225636)
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PurposeHigh Copy number plasmid containing Ple384 (TH MiniPromoter) insert
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC57
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 3518
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePle384
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Alt nameTH MiniPromoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1012
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Entrez GeneTH (a.k.a. DYT14, DYT5b, TYH)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2367 was a gift from Elizabeth Simpson (Addgene plasmid # 225636 ; http://n2t.net/addgene:225636 ; RRID:Addgene_225636) -
For your References section:
MiniPromoters Ple384 (TH) and Ple388 (PITX3) for Targeting Midbrain Dopaminergic Neurons in Mice and Monkeys. Galvan A, Choi D, Korecki AJ, Gomes AdM, Fornes O, Tanimura J, Farkas RA, Lam SL, Lu G, Petkau TL, Yao A, Wasserman WW, Leavitt BR, Simpson EM, and Smith Y.. 2026 Scientific Reports 10.1038/s41598-026-37466-2