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pcDNA3.1-Pur-ottlr3
(Plasmid #225641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225641 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5111
  • Total vector size (bp) 7854
  • Modifications to backbone
    Modified backbone = pcDNA3.1-(-)-Pur (Addgene #200458)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Toll-like receptor 3
  • Alt name
    tlr3
  • Species
    Oncorhynchus tshawytscha
  • Insert Size (bp)
    2743
  • GenBank ID
    XM_024398587
  • Entrez Gene
    tlr3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GGCTAACTAGAGAACCCACTG
  • 3′ sequencing primer GGCAACTAGAAGGCACAGTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert was obtained by gene synthesis (Thermofisher GENART service) and was subloned into pcDNA3.1-Pur.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Pur-ottlr3 was a gift from Bertrand Collet (Addgene plasmid # 225641 ; http://n2t.net/addgene:225641 ; RRID:Addgene_225641)

  • For your References section:

    Toll-like receptor 3 is required for sensing extracellular double stranded RNA in salmonid cells. Collet B, Collins C, Peruzzi M, Catherine-Mezeray J, DeWitte-Orr S, Rebl A, Boudinot P. Marine Biotechnology 2026 10.1007/s10126-026-10590-w
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