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pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP
(Plasmid #226114)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDest
  • Backbone size w/o insert (bp) 7624
  • Total vector size (bp) 7972
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AcrIIC3
  • Species
    Neisseria meningitidis
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP was a gift from Erik Sontheimer (Addgene plasmid # 226114 ; http://n2t.net/addgene:226114 ; RRID:Addgene_226114)

  • For your References section:

    Absolute Quantification of Mammalian MicroRNAs for Therapeutic RNA Cleavage and Detargeting. Kraus C, Wang J, Zheng H, Broderick J, Ajaykumar N, Zamani M, Yang M, Cecchini K, Liang SQ, Kolumba O, Chase K, Lee J, Xue W, Sontheimer E, Gainetdinov I. RNA. 2025 May 28:rna.080566.125. doi: 10.1261/rna.080566.125. 10.1261/rna.080566.125 PubMed 40436630
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