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p-HITI-GFP-Ckm
(Plasmid #226119)

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Item Catalog # Description Quantity Price (USD)
Plasmid 226119 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pZDonor
  • Backbone manufacturer
    Sigma
  • Total vector size (bp) 3963
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ckm
  • gRNA/shRNA sequence
    Unknown
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    719
  • Entrez Gene
    Ckm (a.k.a. CPK-M, Ckmm, M-CK, MCK)
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.18.582796 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-HITI-GFP-Ckm was a gift from Christopher Nelson (Addgene plasmid # 226119 ; http://n2t.net/addgene:226119 ; RRID:Addgene_226119)

  • For your References section:

    Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci. Padmaswari MH, Bulliard G, Agrawal S, Jia MS, Khadgi S, Murach KA, Nelson CE. Mol Ther Nucleic Acids. 2024 Sep 2;35(4):102320. doi: 10.1016/j.omtn.2024.102320. eCollection 2024 Dec 10. 10.1016/j.omtn.2024.102320 PubMed 39398225
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