pAAV_Ckm_F9
(Plasmid
#226140)
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PurposeAAV construct for HITI insert with Ckm gRNA and hF9 transgene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepAAV
- Total vector size (bp) 7192
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameF9
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SpeciesH. sapiens (human)
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Insert Size (bp)2774
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Entrez GeneF9 (a.k.a. F9 p22, FIX, HEMB, P19, PTC, THPH8)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer hU6 promoter (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCkm gRNA
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Alt nameCkm
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SpeciesM. musculus (mouse)
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Entrez GeneCkm (a.k.a. CPK-M, Ckmm, M-CK, MCK)
Gene/Insert 3
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Gene/Insert nameEGFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.18.582796 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_Ckm_F9 was a gift from Christopher Nelson (Addgene plasmid # 226140 ; http://n2t.net/addgene:226140 ; RRID:Addgene_226140) -
For your References section:
Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci. Padmaswari MH, Bulliard G, Agrawal S, Jia MS, Khadgi S, Murach KA, Nelson CE. Mol Ther Nucleic Acids. 2024 Sep 2;35(4):102320. doi: 10.1016/j.omtn.2024.102320. eCollection 2024 Dec 10. 10.1016/j.omtn.2024.102320 PubMed 39398225