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pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD
(Plasmid #226282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226282 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-tight-puro and pLVX-rtTA(advanced)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 9766
  • Total vector size (bp) 11882
  • Modifications to backbone
    Vector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as ISL1:T2A:LHX3 driven by the TREtight promoter
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ISL1:T2A:LHX3
  • Alt name
    ISL1; ISL LIM homeobox 1; Isl-1; ISLET1
  • Alt name
    LHX3; LIM homeobox 3; LIM3
  • Species
    H. sapiens (human)
  • Entrez Gene
    ISL1 (a.k.a. ISLET1, Isl-1)
  • Entrez Gene
    LHX3 (a.k.a. CPHD3, LIM3, M2-LHX3)
  • Tag / Fusion Protein
    • Isl1 and Lhx3 linked via T2A

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGG AGA AGG ATC CTG CCA CCA TGG GAG ACA TG
  • 3′ sequencing primer GGT AGA ATT CAC TCC CTC GAG TTA GAA CTG AGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fred Gage and Chun-Li Zhang.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD was a gift from Susannah Sample (Addgene plasmid # 226282 ; http://n2t.net/addgene:226282 ; RRID:Addgene_226282)

  • For your References section:

    Protocol for direct reprogramming of canine fibroblasts to induced motor neurons. Anderson RS, Corona AC, Suzuki M, Sample SJ. STAR Protoc. 2025 Sep 19;6(3):103975. doi: 10.1016/j.xpro.2025.103975. Epub 2025 Jul 24. 10.1016/j.xpro.2025.103975 PubMed 40711874
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