pRSET A-pcStar
(Plasmid
#226339)
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PurposeBacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepcStar
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SpeciesDerived from Lobophyllia hemprichii
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET A-pcStar was a gift from Pingyong Xu (Addgene plasmid # 226339 ; http://n2t.net/addgene:226339 ; RRID:Addgene_226339) -
For your References section:
Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein. Zhang M, Fu Z, Li C, Liu A, Peng D, Xue F, He W, Gao S, Xu F, Xu D, Yuan L, Zhang F, Xu Z, Xu T, Xu P. Nano Lett. 2020 Apr 8;20(4):2197-2208. doi: 10.1021/acs.nanolett.9b02855. Epub 2019 Oct 7. 10.1021/acs.nanolett.9b02855 PubMed 31576756