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pET28.His.3C.20D3Q.Tau2N4R
(Plasmid #226397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tau2N4R
  • Species
    H. sapiens (human); synthetic
  • Insert Size (bp)
    1376
  • Mutation
    20D3Q, Tau2N4R
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Tag / Fusion Protein
    • His.3C (N terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28.His.3C.20D3Q.Tau2N4R was a gift from Bradley Hyman (Addgene plasmid # 226397 ; http://n2t.net/addgene:226397 ; RRID:Addgene_226397)

  • For your References section:

    Alzheimer disease-associated tau post-translational modification mimics impact tau propagation and uptake. Dickson JR, Sobolewski RGR, Fernandes AR, Cooper JM, Fan Z, Chung M, Donahue C, Oakley DH, Strickland DK, Hyman BT. J Neuropathol Exp Neurol. 2025 Jun 1;84(6):459-470. doi: 10.1093/jnen/nlaf007. 10.1093/jnen/nlaf007 PubMed 39984820
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