TOPO-EXOC3 5' HALF
(Plasmid
#226438)
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PurposeFor subcloning of human EXOC3 promoter (5' half) or for assays using M13 phage
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1-Topo
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEXOC3 5' HALF
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SpeciesH. sapiens (human)
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MutationNo
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Entrez GeneEXOC3 (a.k.a. SEC6, SEC6L1, Sec6p)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS finds 2 single nt mismatches at positions #742 and #803, and a 2 nt deletion at position #469 in the depositor sequence. Depositor confirms that the changes are not expected to impact function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TOPO-EXOC3 5' HALF was a gift from Glen Borchert (Addgene plasmid # 226438 ; http://n2t.net/addgene:226438 ; RRID:Addgene_226438) -
For your References section:
Long G4-rich enhancers target promoters via a G4 DNA-based mechanism. DeMeis JD, Roberts JT, Delcher HA, Godang NL, Coley AB, Brown CL, Shaw MH, Naaz S, Dahal A, Alqudah SY, Nguyen KN, Nguyen AD, Paudel SS, Shell JE, Patil SS, Dang H, O'Neal WK, Knowles MR, Houserova D, Gillespie MN, Borchert GM. Nucleic Acids Res. 2025 Jan 11;53(2):gkae1180. doi: 10.1093/nar/gkae1180. 10.1093/nar/gkae1180 PubMed 39658038