POC1543
(Plasmid
#226484)
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PurposepMOLC360_VR_I9 (Chloramphenicol R) containing the insert I9 to be assembled in POC1519 (pMOBK360_VM) using the site-selective methylation protection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226484 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXLC
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Backbone manufacturerSelf-made
- Backbone size w/o insert (bp) 1820
- Total vector size (bp) 2611
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDNA fragment flanked by BsaI sites
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Alt nameMICA gene fragment modified
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SpeciesH. sapiens (human)
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Insert Size (bp)789
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The DH10B strain should be used for expression of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POC1543 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 226484 ; http://n2t.net/addgene:226484 ; RRID:Addgene_226484)