Skip to main content
Addgene

POC1546
(Plasmid #226487)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    POC1518
  • Backbone manufacturer
    Self-made
  • Backbone size w/o insert (bp) 6402
  • Total vector size (bp) 9889
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
  • Alt name
    MICA gene fragment modified
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3488

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The DH10B strain should be used for expression of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1546 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 226487 ; http://n2t.net/addgene:226487 ; RRID:Addgene_226487)
Related items: