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Addgene

POC1548
(Plasmid #226490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    POC1518
  • Backbone manufacturer
    Self-made
  • Backbone size w/o insert (bp) 6398
  • Total vector size (bp) 7898
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
  • Alt name
    MICA gene fragment modified
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The DH10B strain should be used for expression of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1548 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 226490 ; http://n2t.net/addgene:226490 ; RRID:Addgene_226490)
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