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Addgene

FUS LC 4QQ->AA
(Plasmid #226600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RPIB
  • Backbone size w/o insert (bp) 5223
  • Total vector size (bp) 5780
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    37C for 4 hours
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS LC 4QQ->AA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    557
  • Mutation
    4 QQ pairs replaced with AA pairs at positions 27, 28, 92, 93, 132, 133, 157, 158
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Promoter T7
  • Tag / Fusion Protein
    • 6His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.15.580391 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUS LC 4QQ->AA was a gift from Nicolas Fawzi (Addgene plasmid # 226600 ; http://n2t.net/addgene:226600 ; RRID:Addgene_226600)

  • For your References section:

    Expanding the molecular grammar of polar residues and arginine in FUS phase separation. Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL. Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 10.1038/s41589-024-01828-6. Epub 2025 Feb 7. 10.1038/s41589-024-01828-6 PubMed 39920231
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