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FUS LC noYQ_noYT
(Plasmid #226617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RPIB
  • Backbone size w/o insert (bp) 5223
  • Total vector size (bp) 5780
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS LC noYQ_noYT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    557
  • Mutation
    T7S / Q160S double mutation in LC domain to remove YT or QY sequence pairs
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Promoter T7
  • Tag / Fusion Protein
    • 6His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.15.580391 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUS LC noYQ_noYT was a gift from Nicolas Fawzi (Addgene plasmid # 226617 ; http://n2t.net/addgene:226617 ; RRID:Addgene_226617)

  • For your References section:

    Expanding the molecular grammar of polar residues and arginine in FUS phase separation. Wake N, Weng SL, Zheng T, Wang SH, Kirilenko V, Mittal J, Fawzi NL. Nat Chem Biol. 2025 Jul;21(7):1076-1088. doi: 10.1038/s41589-024-01828-6. Epub 2025 Feb 7. 10.1038/s41589-024-01828-6 PubMed 39920231
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