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Addgene

pGGG L2 TaStb15
(Plasmid #226630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226630 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGoldenGreenGate (pGGG)
  • Backbone manufacturer
    Mark Smedley - John Innes Centre, UK
  • Backbone size w/o insert (bp) 10483
  • Total vector size (bp) 16560
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Triticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene
  • Species
    Triticum aestivum (wheat)
  • Insert Size (bp)
    6077
  • Promoter Native TaStb15

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer NGS
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol 38, 1274–1279 (2020). We acknowledge the researchers at UC Davis and HHMI for the development of GRF4-GIF1 fusion reported in PMID: 33046875.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: For replication in Agrobacterium, pGGG derivatives require the pSOUP helper plasmid Addgene #165419.

Please visit https://doi.org/10.1101/2023.09.11.557217 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGG L2 TaStb15 was a gift from Brande Wulff (Addgene plasmid # 226630 ; http://n2t.net/addgene:226630 ; RRID:Addgene_226630)

  • For your References section:

    Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase. Hafeez AN, Chartrain L, Feng C, Cambon F, Clarke M, Griffiths S, Hayta S, Jiang M, Keller B, Kirby R, Kolodziej MC, Powell OR, Smedley MA, Steuernagel B, Xian W, Wingen LU, Cheng S, Saintenac C, Wulff BBH, Brown JKM. Nat Plants. 2025 Mar;11(3):410-420. doi: 10.1038/s41477-025-01920-2. Epub 2025 Mar 14. 10.1038/s41477-025-01920-2 PubMed 40087541
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