pCMV6-CNDP2-E166D
(Plasmid
#226720)
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PurposeMammalian expression of cytosolic mouse CNDP2 mutant E166D
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6-Entry
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCNDP2
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SpeciesM. musculus (mouse)
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MutationE166D
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Entrez GeneCndp2 (a.k.a. 0610010E05Rik, Cn2, Dip-2, Pep-1, Pep1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SgfI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer VP1.5
- 3′ sequencing primer XL39 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6-CNDP2-E166D was a gift from Jonathan Long (Addgene plasmid # 226720 ; http://n2t.net/addgene:226720 ; RRID:Addgene_226720) -
For your References section:
A beta-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites. Moya-Garzon MD, Wang M, Li VL, Lyu X, Wei W, Tung AS, Raun SH, Zhao M, Coassolo L, Islam H, Oliveira B, Dai Y, Spaas J, Delgado-Gonzalez A, Donoso K, Alvarez-Buylla A, Franco-Montalban F, Letian A, Ward CP, Liu L, Svensson KJ, Goldberg EL, Gardner CD, Little JP, Banik SM, Xu Y, Long JZ. Cell. 2025 Jan 9;188(1):175-186.e20. doi: 10.1016/j.cell.2024.10.032. Epub 2024 Nov 12. 10.1016/j.cell.2024.10.032 PubMed 39536746