Skip to main content
Addgene

pBAD-WzxE-TEV-His10
(Plasmid #226781)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD24
  • Backbone size w/o insert (bp) 4617
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    protein expression in E. coli C43(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wzxE
  • Species
    Escherichia coli
  • Insert Size (bp)
    1250
  • Mutation
    valine insertion in position 2 due to the cloning strategy
  • GenBank ID
  • Promoter araBAD
  • Tag / Fusion Protein
    • TEV cleavable 10xHistidine tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTTTGCTATGCCATAGC
  • 3′ sequencing primer TTTCACTTCTGAGTTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-WzxE-TEV-His10 was a gift from James Naismith (Addgene plasmid # 226781 ; http://n2t.net/addgene:226781 ; RRID:Addgene_226781)

  • For your References section:

    Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide. Le Bas A, Clarke BR, Teelucksingh T, Lee M, El Omari K, Giltrap AM, McMahon SA, Liu H, Beale JH, Mykhaylyk V, Duman R, Paterson NG, Ward PN, Harrison PJ, Weckener M, Pardon E, Steyaert J, Liu H, Quigley A, Davis BG, Wagner A, Whitfield C, Naismith JH. Open Biol. 2025 Jan;15(1):240310. doi: 10.1098/rsob.240310. Epub 2025 Jan 8. 10.1098/rsob.240310 PubMed 39772807
Related items: