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Addgene

pTT021
(Plasmid #226782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wzxE, wecF, wzyE, wecG
  • Species
    Escherichia coli
  • Insert Size (bp)
    4509
  • Promoter BAD

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: genes were amplified from E. coli K-12 Sphi874

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTT021 was a gift from James Naismith & Chris Whitfield (Addgene plasmid # 226782 ; http://n2t.net/addgene:226782 ; RRID:Addgene_226782)

  • For your References section:

    Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide. Le Bas A, Clarke BR, Teelucksingh T, Lee M, El Omari K, Giltrap AM, McMahon SA, Liu H, Beale JH, Mykhaylyk V, Duman R, Paterson NG, Ward PN, Harrison PJ, Weckener M, Pardon E, Steyaert J, Liu H, Quigley A, Davis BG, Wagner A, Whitfield C, Naismith JH. Open Biol. 2025 Jan;15(1):240310. doi: 10.1098/rsob.240310. Epub 2025 Jan 8. 10.1098/rsob.240310 PubMed 39772807
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