pCambia1300-NP-PTII
(Plasmid
#226816)
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Purpose(Empty Backbone) Efficient screening of gene editing in plants (kanamycin resistance).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCambia1300
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Vector typePlant Expression, CRISPR
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Selectable markersSplit NPTII gene for kanamycin resistance
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCambia1300-NP-PTII was a gift from Daisuke Miki (Addgene plasmid # 226816 ; http://n2t.net/addgene:226816 ; RRID:Addgene_226816) -
For your References section:
SSA-mediated selection marker gene activation enhances relative gene-targeting efficiency in plants. Kong D, Cheng Y, Ke Y, Dang X, Liu X, Wang C, Huang C, Ye R, Miki D. Horticulture Research, 06 Aug 2025, uhaf196 10.1093/hr/uhaf196