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pISEre1
(Plasmid #226819)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226819 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ISEre1
  • Species
    [Eubacterium] rectale strain AM42-1

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a M140T mutation in Exo. This mutation is not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISEre1 was a gift from Sheng Yang (Addgene plasmid # 226819 ; http://n2t.net/addgene:226819 ; RRID:Addgene_226819)
  • For your References section:

    Implementation of RAGATH RNA-associated DNA Endonucleases as Genome Editing Tool in Escherichia coli. Zhou X, Yang S, Sun B, Dong F, Yin M, Jiang Y, Huang Z, Yang S. Biotechnol J. 2025 Mar;20(3):e70005. doi: 10.1002/biot.70005. 10.1002/biot.70005 PubMed 40111000