Skip to main content
Addgene

pSIG1
(Plasmid #226908)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226908 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pICH47732
  • Backbone manufacturer
    MoClo Toolkit
  • Backbone size (bp) 4376
  • Modifications to backbone
    Add two T7 promoters and terminators, two BsmBI sites, and a negative selection marker, sacB gene.
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter T7 promoter
  • Selectable markers
    sacB (select with 10% sucrose)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sylvestre Marillonnet (Addgene plasmid # 48000).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pSIG plasmids are derived from the level 1 plasmid pICH47732 (addgene #48000) within the MoClo toolkit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIG1 was a gift from Li Hung Chen (Addgene plasmid # 226908 ; http://n2t.net/addgene:226908 ; RRID:Addgene_226908)

  • For your References section:

    pSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain. Wu C-F, Chang L-P, Lee C, Stergiopoulos I, Chen L-H. Plant Methods 21, 96 (2025). https://doi.org/10.1186/s13007-025-01413-5 10.1186/s13007-025-01413-5
Related items: