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Addgene

pPSmF
(Plasmid #226912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPSup
  • Backbone manufacturer
    Jores et al, 2020
  • Backbone size (bp) 5464
  • Modifications to backbone
    Deleted upstream BlpR cassette and ~300bp upstream of the RB T-DNA repeat
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSmF was a gift from Stan Fields (Addgene plasmid # 226912 ; http://n2t.net/addgene:226912 ; RRID:Addgene_226912)

  • For your References section:

    Small DNA elements can act as both insulators and silencers in plants. Jores T, Mueth NA, Tonnies J, Char SN, Liu B, Grillo-Alvarado V, Abbitt S, Anand A, Deschamps S, Diehn S, Gordon-Kamm B, Jiao S, Munkvold K, Snowgren H, Sardesai N, Fields S, Yang B, Cuperus JT, Queitsch C. Plant Cell. 2025 Jun 4;37(6):koaf084. doi: 10.1093/plcell/koaf084. 10.1093/plcell/koaf084 PubMed 40485594
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