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CPH3737_GST-TEV-Spt6p-tSH2-1223-1453
(Plasmid #227087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST15
  • Backbone size w/o insert (bp) 5429
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spt6-tsh2
  • Alt name
    YGR116W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    688
  • Mutation
    K1355A, K1435A
  • Entrez Gene
    SPT6 (a.k.a. YGR116W, CRE2, SSN20)
  • Promoter T7
  • Tag / Fusion Protein
    • GST-TEV_cleavage_site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a V1480M mutation in the spt6 tsh2 domain. It is not known how this mutation affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CPH3737_GST-TEV-Spt6p-tSH2-1223-1453 was a gift from Chris Hill (Addgene plasmid # 227087 ; http://n2t.net/addgene:227087 ; RRID:Addgene_227087)
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