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pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his
(Plasmid #227163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227163 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCS2+8
  • Backbone size w/o insert (bp) 4134
  • Total vector size (bp) 6617
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYP2A6
  • Alt name
    Cytochrome P450 2A6
  • Species
    H. sapiens (human)
  • Mutation
    changed cysteine 82 and cysteine 439 to alanine
  • GenBank ID
    NM_000762.6
  • Entrez Gene
    CYP2A6 (a.k.a. CPA6, CYP2A, CYP2A3, CYPIIA6, P450C2A, P450PB)
  • Promoter CMV and SP6
  • Tags / Fusion Proteins
    • Flag (C terminal on insert)
    • HaloTag (C terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV forward or SP6 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his was a gift from Yimon Aye (Addgene plasmid # 227163 ; http://n2t.net/addgene:227163 ; RRID:Addgene_227163)

  • For your References section:

    Organ-specific electrophile responsivity mapping in live C. elegans. Liu J, Kulkarni A, Gao YQ, Urul DA, Hamelin R, Novotny BA, Long MJC, Aye Y. Cell. 2024 Dec 26;187(26):7450-7469.e29. doi: 10.1016/j.cell.2024.10.014. Epub 2024 Nov 5. 10.1016/j.cell.2024.10.014 PubMed 39504959
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