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pWormgate2-myo-2p-gfp-halo
(Plasmid #227169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227169 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pWormgate2
  • Backbone size w/o insert (bp) 3276
  • Total vector size (bp) 6034
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends XL10-Gold strain for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Promoter myo-2
  • Tag / Fusion Protein
    • Halotag (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWormgate2-myo-2p-gfp-halo was a gift from Yimon Aye (Addgene plasmid # 227169 ; http://n2t.net/addgene:227169 ; RRID:Addgene_227169)

  • For your References section:

    Organ-specific electrophile responsivity mapping in live C. elegans. Liu J, Kulkarni A, Gao YQ, Urul DA, Hamelin R, Novotny BA, Long MJC, Aye Y. Cell. 2024 Dec 26;187(26):7450-7469.e29. doi: 10.1016/j.cell.2024.10.014. Epub 2024 Nov 5. 10.1016/j.cell.2024.10.014 PubMed 39504959
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