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pAAV-hSyn1-DIO-SEP-GluA1
(Plasmid #227235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227235 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 3996
  • Total vector size (bp) 7464
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow at 30C for a short time to avoid ITR deletions
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEP-GluA1
  • Alt name
    superecliptic pHluorin-tagged AMPA receptor GluA1 subunit
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3303
  • Entrez Gene
    Gria1 (a.k.a. GluA1, gluR-A)
  • Promoter hSyn1
  • Tag / Fusion Protein
    • superecliptic pHluorin (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    superecliptic pHluorin was based on Transmission of Olfactory Information between Three Populations of Neurons in the Antennal Lobe of the Fly. Ng M, Roorda Rd, Lima Sq, Zemelman Bv, Morcillo P, Miesenböck G. (2002). Neuron, 36(3) , 463-474. doi: 10.1016/s0896-6273(02)00975-3.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.07.20.549908 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1-DIO-SEP-GluA1 was a gift from Richard Huganir (Addgene plasmid # 227235 ; http://n2t.net/addgene:227235 ; RRID:Addgene_227235)

  • For your References section:

    Calcium-permeable AMPA receptors govern PV neuron feature selectivity. Hong I, Kim J, Hainmueller T, Kim DW, Keijser J, Johnson RC, Park SH, Limjunyawong N, Yang Z, Cheon D, Hwang T, Agarwal A, Cholvin T, Krienen FM, McCarroll SA, Dong X, Leopold DA, Blackshaw S, Sprekeler H, Bergles DE, Bartos M, Brown SP, Huganir RL. Nature. 2024 Nov;635(8038):398-405. doi: 10.1038/s41586-024-08027-2. Epub 2024 Oct 2. 10.1038/s41586-024-08027-2 PubMed 39358515
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