Skip to main content
Addgene
Open Menu

pAAV-hSyn1-DIO-eGFP
(Plasmid #227237)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227237 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4075
  • Total vector size (bp) 4792
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow at 30C for a short time to avoid ITR deletions
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    Enhanced Green Fluorescent Protein
  • Insert Size (bp)
    717
  • Entrez Gene
    eGFP (a.k.a. pPRS3a_01)
  • Promoter hSyn1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.07.20.549908 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1-DIO-eGFP was a gift from Richard Huganir (Addgene plasmid # 227237 ; http://n2t.net/addgene:227237 ; RRID:Addgene_227237)

  • For your References section:

    Calcium-permeable AMPA receptors govern PV neuron feature selectivity. Hong I, Kim J, Hainmueller T, Kim DW, Keijser J, Johnson RC, Park SH, Limjunyawong N, Yang Z, Cheon D, Hwang T, Agarwal A, Cholvin T, Krienen FM, McCarroll SA, Dong X, Leopold DA, Blackshaw S, Sprekeler H, Bergles DE, Bartos M, Brown SP, Huganir RL. Nature. 2024 Nov;635(8038):398-405. doi: 10.1038/s41586-024-08027-2. Epub 2024 Oct 2. 10.1038/s41586-024-08027-2 PubMed 39358515
Related items: