Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22728||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1252
Entrez GeneTrp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
- Cloning method Restriction Enzyme
- 5′ cloning site attB1 (unknown if destroyed)
- 3′ cloning site attB2 (unknown if destroyed)
- 5′ sequencing primer pMX-S1811
- 3′ sequencing primer pMXs-AS3200 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
additional article reference: Cecchinelli, B. et al. Ser58 of mouse p53 is the homologue of human Ser46 and is phosphorylated by HIPK2 in apoptosis. Cell Death Differ. 2006 Nov;13(11):1994-7. Epub 2006 May 26.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-p53S58A was a gift from Shinya Yamanaka (Addgene plasmid # 22728 ; http://n2t.net/addgene:22728 ; RRID:Addgene_22728)
For your References section:Suppression of induced pluripotent stem cell generation by the p53-p21 pathway. Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, Okita K, Yamanaka S. Nature. 2009 Aug 27. 460(7259):1132-5. 10.1038/nature08235 PubMed 19668191
Map uploaded by the depositor.