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Addgene

pk335-CARGO-6mer-55kb-USF
(Plasmid #227458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pk335-BFP
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418) ; TagBFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARGO to 55kb Upstream Fgf5
  • Alt name
    CARGO12
  • gRNA/shRNA sequence
    TCCGTAAGAGTAAGGTGACC, ATATCCAGATAGAAGCTGCG, CTTACTAGCGCTGACCTTTA, TCCCTGGTCTATGGTAAGAA, TTTAAAAAGCCTCCCCTTTC, CCGCCCTCTATGGTTAAGTA
  • Species
    M. musculus (mouse), Synthetic

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pk335-CARGO-6mer-55kb-USF was a gift from Joanna Wysocka (Addgene plasmid # 227458 ; http://n2t.net/addgene:227458 ; RRID:Addgene_227458)

  • For your References section:

    Long-range regulation of transcription scales with genomic distance in a gene-specific manner. Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell JE Jr, Boettiger AN, Wysocka J. Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2. 10.1016/j.molcel.2024.10.021 PubMed 39626660
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