pCLJ-Fgf5-t2A-eGFP
(Plasmid #227504)

• Purpose: Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette
• Depositing Lab: Joanna Wysocka
• Publication: Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: pAAV
• Vector type: AAV, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: M. musculus (mouse), Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pCLJ-Fgf5-t2A-eGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UBC-NeoR selection cassette flanked by LoxP sites