pEPMS0CM0042
(Plasmid
#227524)
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PurposeL0 part - CDS (without STOP codon)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepTwist Chlor High Copy
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYP716A431 (Calendula officinalis cytochrome P450 5)
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SpeciesCalendula officinalis
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MutationBsaI sites removed by silent mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPMS0CM0042 was a gift from Nicola Patron (Addgene plasmid # 227524 ; http://n2t.net/addgene:227524 ; RRID:Addgene_227524) -
For your References section:
Biosynthesis and bioactivity of anti-inflammatory triterpenoids in Calendula officinalis. Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, Schudoma C, Swarbreck D, O'Connell MA, Patron NJ. Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w. 10.1038/s41467-025-62269-w PubMed 40721615