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Addgene

pEPDG1CB0028
(Plasmid #227525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227525 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist Chlor High Copy
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYP716A392a (Calendula arvensis cytochrome P450 1)
  • Species
    Calendula arvensis
  • Mutation
    BsaI sites removed by silent mutation

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains the domestication mutation F470L mutation in CYP716A392a.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPDG1CB0028 was a gift from Nicola Patron (Addgene plasmid # 227525 ; http://n2t.net/addgene:227525 ; RRID:Addgene_227525)

  • For your References section:

    Biosynthesis and bioactivity of anti-inflammatory triterpenoids in Calendula officinalis. Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, Schudoma C, Swarbreck D, O'Connell MA, Patron NJ. Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w. 10.1038/s41467-025-62269-w PubMed 40721615
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