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Addgene

pEPHS1CB0020
(Plasmid #227564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47732
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35Sshort_TMV_CoTXSS (G380T/D385E/H492Q/P751A)_35S
  • Species
    Calendula officinalis
  • Mutation
    G380T / D385E / H492Q / P751A, BsaI sites removed by silent mutation
  • Promoter 35Sshort_TMV (pICH51277)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPHS1CB0020 was a gift from Nicola Patron (Addgene plasmid # 227564 ; http://n2t.net/addgene:227564 ; RRID:Addgene_227564)

  • For your References section:

    Biosynthesis and bioactivity of anti-inflammatory triterpenoids in Calendula officinalis. Patron N. Nature Communications 2025 10.17863/CAM.119887
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