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pLKO.1-shAtg7-EGFP
(Plasmid #227684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227684 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLKO.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg7 shRNA
  • gRNA/shRNA sequence
    TATTATTGAGTTCAGAGCTGG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Atg7 (a.k.a. 1810013K23Rik, Agp7, Apg7l, Atg7l, Gm21553)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.09.536178 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1-shAtg7-EGFP was a gift from Cagla Eroglu (Addgene plasmid # 227684 ; http://n2t.net/addgene:227684 ; RRID:Addgene_227684)

  • For your References section:

    PD-linked LRRK2 G2019S mutation impairs astrocyte morphology and synapse maintenance via ERM hyperphosphorylation. Wang S, Baumert R, Séjourné G, Bindu DS, Dimond K, Sakers K, Vazquez L, Moore JL, Tan CX, Takano T, Rodriguez MP, Brose N, Bradley L, Lessing R, Soderling SH, La Spada AR, Eroglu C. eLife 2025 [Reviewed Preprint] 14:RP107556. doi: https://doi.org/10.7554/eLife.107556.1 10.7554/eLife.107556.1
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