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Addgene

pGEX GAC F7
(Plasmid #227829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227829 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEX
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAC isoform of F7
  • Species
    H. sapiens (human)
  • Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K176, E177, D180, Q187, V209, T212, Q213, R216, K218, D223, S226, H230, F439, G444, E445, R446, V447, P450, R534, H535, F536, K538, L540, R544, and E545 to A
  • Entrez Gene
    GLS (a.k.a. AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • GST (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX GAC F7 was a gift from Sheng-cai Lin (Addgene plasmid # 227829 ; http://n2t.net/addgene:227829 ; RRID:Addgene_227829)

  • For your References section:

    AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine. Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu L, Piao HL, Deng X, Han J, Zhang CS, Lin SC. Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6. 10.1038/s41422-024-00985-6 PubMed 38898113
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