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pET-28a PDZD8-T527D
(Plasmid #227851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDZD8
  • Species
    H. sapiens (human)
  • Mutation
    T527D
  • Entrez Gene
    PDZD8 (a.k.a. IDDADF, PDZK8)
  • Tag / Fusion Protein
    • HIS (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a PDZD8-T527D was a gift from Sheng-cai Lin (Addgene plasmid # 227851 ; http://n2t.net/addgene:227851 ; RRID:Addgene_227851)

  • For your References section:

    AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine. Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu L, Piao HL, Deng X, Han J, Zhang CS, Lin SC. Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6. 10.1038/s41422-024-00985-6 PubMed 38898113
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