pAAV-hSyn-Gluc-NHP.RMA-IRES-EGFP
(Plasmid #227886)

• Purpose: Expresses Gluc-NHP.RMA and EGFP under the hSyn promoter. Gluc-NHP.RMA for monitoring neuronal transduction.
• Depositing Lab: Jerzy Szablowski
• Publication: Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/2025.06.01.657212

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4569
• Total vector size (bp): 7126
• Vector type: Mammalian Expression, AAV, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase fused to macaque Fc
• Alt name: Gluc-macaque IgG1 Fc
• Alt name: Gluc-NHP.RMA
• Species: Synthetic; Gaussia princeps, Macaca mulatta
• GenBank ID: ATV90900.1
• Tags / Fusion Proteins:
  • Gluc (N terminal on insert)
  • IgG1 Fc (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcagcgctgcctcagtct

Gene/Insert 2

• Gene/Insert name: IRES-EGFP

Resource Information

• Supplemental Documents:
  • psl17-1_paav-hsyn-glucm23-nhp-igg1-fc-ires-egfp.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gluc is derived from AAV-hSyn-GlucM23-iChloC-EYFP (Addgene #114102).
IRES-EGFP is derived from pAAV.CMV.Luc.IRES.EGFP.SV40 (Addgene #105533).

Please visit https://doi.org/10.1101/2025.06.01.657212 for bioRxiv preprint.