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Addgene

YEE_GFP
(Plasmid #228184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK095
  • Backbone manufacturer
    John Dueber, Addgene plasmid #65202
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Empty vector for protein expression in S. cerevisiae.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YEE_GFP was a gift from Joseph Brock (Addgene plasmid # 228184 ; http://n2t.net/addgene:228184 ; RRID:Addgene_228184)

  • For your References section:

    High-throughput optimisation of protein secretion in yeast via an engineered biosensor. Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J. Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13. 10.1016/j.tibtech.2024.11.010 PubMed 39674781
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