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Addgene

SP_G6
(Plasmid #228186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber, Addgene plasmid #65108
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sp_Q6UW88_EPGN_HUMAN:0-20
  • Alt name
    Uniprot: Q6UW88
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Signal peptides direct recombinant protein secretion in yeast. Codon optimised for expression in S. cerevisiae. This part is designed for use with components from the MoClo Yeast Toolkit (Lee et al. 2015). This insert is a 3a* component, see Williams et al. 2024 for more detail.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP_G6 was a gift from Joseph Brock (Addgene plasmid # 228186 ; http://n2t.net/addgene:228186 ; RRID:Addgene_228186)

  • For your References section:

    High-throughput optimisation of protein secretion in yeast via an engineered biosensor. Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J. Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13. 10.1016/j.tibtech.2024.11.010 PubMed 39674781
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