pACE-MBP-FBXL17 (310–701)
(Plasmid
#228369)
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Purposeexpress human FBXL17 in insect cells, such as Trichoplusia ni Hi5
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACE
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFBXL17
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SpeciesH. sapiens (human)
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Entrez GeneFBXL17 (a.k.a. FBXO13, Fbl17, Fbx13)
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Tag
/ Fusion Protein
- MBP
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer aaaacctctacttccaatcG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACE-MBP-FBXL17 (310–701) was a gift from Ning Zheng (Addgene plasmid # 228369 ; http://n2t.net/addgene:228369 ; RRID:Addgene_228369) -
For your References section:
Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress. Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N. Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012. 10.1016/j.cell.2024.10.012 PubMed 39504958