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synapsin-hIRβ-mCherry
(Plasmid #228449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR-SFFV-KRAB-dCas9-P2A-mCherry
  • Modifications to backbone
    They contain the neuron-specific synapsin promoter as well as a new marker, mCherry. The self-cleaving P2A site preceding the mCherry sequence produces mCherry expression at a 1:1 ratio with the IRβ protein, thus improving fluorescence. All current work on the IRβ protein uses either lentiviruses or AAVs based on this plasmid set, as the mCherry marker has shown robust expression both in vitro and in vivo and the synapsin promoter shows no detectable expression in astrocytes.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Insulin Receptor beta subunit
  • Alt name
    hIR beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1404
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)
  • Promoter neuron-specific synapsin promote

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    synapsin-hIRβ-mCherry was a gift from Olivier Thibault (Addgene plasmid # 228449 ; http://n2t.net/addgene:228449 ; RRID:Addgene_228449)

  • For your References section:

    Expression of a Constitutively Active Human Insulin Receptor in Hippocampal Neurons Does Not Alter VGCC Currents. Frazier HN, Anderson KL, Maimaiti S, Ghoweri AO, Kraner SD, Popa GJ, Hampton KK, Mendenhall MD, Norris CM, Craven RJ, Thibault O. Neurochem Res. 2019 Jan;44(1):269-280. doi: 10.1007/s11064-018-2510-2. Epub 2018 Mar 23. 10.1007/s11064-018-2510-2 PubMed 29572644
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